Open AccessImaging mass spectrometry: a new tool for kidney disease investigations
Author(s) -
Maciej Łałowski,
Fulvio Magni,
Veronica Mainini,
Evanthia Monogioudi,
Athanasios Gotsopoulos,
Rabah Soliymani,
Clizia Chinello,
Marc Baumann
Publication year - 2013
Publication title -
nephrology dialysis transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.654
H-Index - 168
eISSN - 1460-2385
pISSN - 0931-0509
DOI - 10.1093/ndt/gft008
Subject(s) - mass spectrometry imaging , mass spectrometry , medicine , maldi imaging , proteomics , biomarker discovery , computational biology , molecular imaging , profiling (computer programming) , matrix assisted laser desorption/ionization , pathology , chromatography , chemistry , biochemistry , in vivo , biology , desorption , computer science , microbiology and biotechnology , organic chemistry , adsorption , gene , operating system
Matrix-assisted laser desorption ionization (MALDI)-profiling and imaging mass spectrometry are promising technologies for measuring hundreds of different molecules directly on tissues. For instance, small molecules, drugs and their metabolites, endogenous lipids, carbohydrates and complex peptides/proteins can be measured at the same time without significant disruption of sample integrity. In this review, the potential of MALDI-profiling/imaging technologies in disease proteomics, drug action and studies of cellular processes in the context of kidney tissue is described. Spatial and sequence information obtained in tissue MALDI-profiling/imaging studies can be correlated with other mass spectrometry-based techniques, auxiliary imaging technologies and routine (immuno) histochemical staining.
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