Open AccessDetection of anti-ESA antibodies in human samples from PRCA and non-PRCA patients: an immunoassay platform comparison
Author(s) -
Troy E. Barger,
Andrew J. Kuck,
Narendra Chirmule,
Steven J. Swanson,
Daniel T. Mytych
Publication year - 2011
Publication title -
nephrology dialysis transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.654
H-Index - 168
eISSN - 1460-2385
pISSN - 0931-0509
DOI - 10.1093/ndt/gfr213
Subject(s) - polyclonal antibodies , medicine , antibody , immunoassay , immunology
The immunological methods for detecting antibodies to erythropoiesis-stimulating agents (ESAs) differ in assay sensitivity. However, this parameter, routinely determined in clinical assays using a high-affinity non-human polyclonal antibody, gives a one-dimensional assessment of antibody detection. We compare three widely used immunological methods and evaluate the ability of each to detect mature human antibodies and human antibodies characteristic of an early immune response.
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