Open AccessApoptosis and necrosis during ischaemia in renal tubular cells (LLC-PK1 and MDCK)
Author(s) -
Gerd Wiegele,
M. Brandis,
Lothar Bernd Zimmerhackl
Publication year - 1998
Publication title -
nephrology, dialysis, transplantation/nephrology dialysis transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.654
H-Index - 168
eISSN - 1460-2385
pISSN - 0931-0509
DOI - 10.1093/ndt/13.5.1158
Subject(s) - apoptosis , propidium iodide , trypan blue , necrosis , programmed cell death , medicine , fragmentation (computing) , dna fragmentation , viability assay , intracellular , ischemia , microbiology and biotechnology , pathology , biology , biochemistry , ecology
Ischaemia is the most frequent cause of acute renal failure. It has been previously demonstrated that ischaemia is connected with signs of necrosis and apoptosis. Apoptosis is an energy-dependent process. During ischaemia intercellular energy levels decline rapidly. Therefore, the goal of the investigation was to reveal the time dependency of cell death mechanisms during ischaemia leading to irreversibility of tissue damage.