Open AccessCharacterization of heme coordination structure in heme-DNA complex possessing gaseous molecule as an exogenous ligand
Author(s) -
Kaori Saito,
Yusuke Nakano,
Hulin Tai,
Shigenori Nagatomo,
Hikaru Hemmi,
Hajime Mita,
Yasuhiko Yamamoto
Publication year - 2009
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrp121
Subject(s) - heme , myoglobin , hemeprotein , dna , chemistry , ligand (biochemistry) , molecule , protoporphyrin , biochemistry , stereochemistry , porphyrin , enzyme , receptor , organic chemistry
We have previously demonstrated that heme, iron(III)-protoporphyrin IX complex, and a parallel-quadruplex DNA assembled from d(TTAGGG) form a stable coordination complex called "heme-DNA complex". The heme-DNA complex exhibits a variety of spectroscopic characteristics remarkably similar to those of met-form of myoglobin, oxygen-binding hemoprotein, reflecting that the heme environments in the two systems are highly alike to each other. In a course of our effort toward exploring functional properties of the heme-DNA complex, we have investigated binding of gaseous molecules such as CO and O(2) to the heme-DNA complex. The present study revealed that the heme-DNA complex exhibits ability to accommodate these molecules as exogenous ligands.