Open AccessDevelopment of a rapid and reversible photocrosslinking of RNA
Author(s) -
Y. Yoshimura,
Tsuyoshi Ohtake,
Hajime Okada,
Kenzo Fujimoto
Publication year - 2009
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrp099
Subject(s) - rna , nucleoside , pyrimidine , chemistry , yield (engineering) , residue (chemistry) , combinatorial chemistry , biophysics , stereochemistry , biology , biochemistry , materials science , gene , metallurgy
We describe a novel reversible interstrand photocrosslinking reaction of RNA. In this system, a modified oligodeoxynucleotide (ODN) containing 3-cyanovinylcarbazole nucleoside ((CNV)K) reacts by photoirradiation at 366 nm for 1 s with pyrimidine residue of a complementary template RNA to yield a crosslinked product in 94% yield.
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