Improvement of transcription- and transfection-efficiency by synthetic polyampholytes | Zendy

Chieko Yoshihara | Zendy; Tomoko Ito | Zendy; ChwenYang Shew | Zendy; Yoshiyuki Koyama | Zendy

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Improvement of transcription- and transfection-efficiency by synthetic polyampholytes

Author(s) -

Chieko Yoshihara,

Tomoko Ito,

ChwenYang Shew,

Yoshiyuki Koyama

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn360

Subject(s) - polyethylenimine , transfection , transcriptional activity , chemistry , transcription (linguistics) , dna , peg ratio , transgene , transcription factor , biophysics , gene delivery , microbiology and biotechnology , gene , biochemistry , biology , linguistics , philosophy , finance , economics

Water-soluble PEG derivatives having both amino- and carboxyl-pendants (PEG-ACs) were synthesized, and examined for their transcription- and transfection-enhancing activity on DNA/polycation complexes. PEG-AC could be deposited onto the surface of DNA/polyethylenimine(PEI) complexes, and enhanced their transcriptional activity. Fluorescence anisotropy study showed that the amphoteric PEG-AC loosened the tightly compacted DNA/PEI complex to facilitate the approach of transcriptional factors. Transcriptional activity of the complex was in good correlation with its transgene expression efficiency, indicating the importance of improvement in transcription for effective gene transfection.

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