Functionalization of DNA G-Wires for patterning and nanofabrication | Zendy

Sébastien Lyonnais | Zendy; Olivier Piétrement | Zendy; A. Chepelianski | Zendy; S. Guéron | Zendy; Laurent Lacroix | Zendy; Éric Le Cam | Zendy; JeanLouis Mergny | Zendy

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Functionalization of DNA G-Wires for patterning and nanofabrication

Author(s) -

Sébastien Lyonnais,

Olivier Piétrement,

A. Chepelianski,

S. Guéron,

Laurent Lacroix,

Éric Le Cam,

JeanLouis Mergny

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn348

Subject(s) - klenow fragment , nanolithography , surface modification , template , nanotechnology , dna origami , dna , materials science , guanine , chemistry , polymerase , nanostructure , nucleotide , fabrication , medicine , biochemistry , alternative medicine , pathology , exonuclease , gene

DNA structures made of guanine tetrads present remarkable properties and are thus first choice candidates for applications in nanofabrication. Starting from the work of Kotlyar et al., we report here that the klenow exo(-) fragment of DNA polymerase I can extend poly(dG)-poly(dC) from various 5'-modified (dG)(10(-))(dC)(10) templates. This allows the production of end-functionalized four-stranded wires (G-Wires) assembled from the folding of poly(dG) strands. G-Wires bearing thiol moieties can be easily combed on Au and Pt surfaces, whereas a 5' single-stranded overhang of a random sequence provides the unique possibility to assemble complex structures for nanoconstruction purposes.

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