On the analysis of fluorimetric titration curves of purine nucleoside phosphorylase | Zendy

Jan Antosiewicz | Zendy; Katarzyna Breer | Zendy; Agnieszka Bzowska | Zendy; Beata WielgusKutrowska | Zendy

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On the analysis of fluorimetric titration curves of purine nucleoside phosphorylase

Author(s) -

Jan Antosiewicz,

Katarzyna Breer,

Agnieszka Bzowska,

Beata WielgusKutrowska

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn339

Subject(s) - purine nucleoside phosphorylase , titration , chemistry , nucleoside , purine , titration curve , biochemistry , molecule , glycogen phosphorylase , stereochemistry , enzyme , organic chemistry

The steady-state fluorimetric titration curves for trimeric purine nucleoside phosphorylase (PNP) by two ligands, were analysed using the DynaFit program. Results of this analysis indicate that three binding sites of PNP molecule interact with each other and that the character of this interaction is different for both ligands. The DynaFit program is very useful in studies of oligomeric proteins, but for detection of non-interacting sites some independent tests are necessary.

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