Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme | Zendy

Junya Ishikawa | Zendy; Hiroyuki Furuta | Zendy; Yoshiya Ikawa | Zendy

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Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme

Author(s) -

Junya Ishikawa,

Hiroyuki Furuta,

Yoshiya Ikawa

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn265

Subject(s) - ribozyme , digital subscriber line , vs ribozyme , base pair , hairpin ribozyme , ligase ribozyme , modular design , base (topology) , computational biology , genetics , biology , rna , computer science , dna , mathematics , programming language , computer network , mathematical analysis , gene

The class DSL ribozyme is one of artificial RNA enzymes generated by module-based molecular design. In the structure of this ribozyme, two most important functional modules are connected by a U-A base-pair. We have examined the possible importance of this base-pair by site-directed mutation experiments using the DSL-1S ribozyme and its derivative possessing altered modular organization. The analysis indicated that the DSL-1S ribozyme preferred U-A pair at the positions whereas the derivative preferred A-U pair.

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