Open AccessOligonucleotides damaged with Carcinogenic Aromatic Amines at the C8- and N6-Position of 2'-Deoxyadenosine
Author(s) -
M. I. Jacobsen,
Chris Meier
Publication year - 2008
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrn223
Subject(s) - deoxyadenosine , oligonucleotide , carcinogen , chemistry , biochemistry , dna
The synthesis of C8- and N(6)-adducts of 2'- deoxyadenosine with carcinogenic aromatic amines is described. Furthermore, the adducts were converted into their corresponding phosphoramidites. These were applied to solid phase DNA synthesis to obtain the site-specifically modified oligonucleotides that were characterized and investigated regarding melting temperature, circular dichroism spectra and in some cases by enzymatic degradation assays.
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