Open AccessDevelopment of template-directed reversible DNA photocrosslinking
Author(s) -
Y. Yoshimura,
Guilherme Akio Tamura Ozaki,
Kenzo Fujimoto
Publication year - 2008
Publication title -
nucleic acids symposium series
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrn215
Subject(s) - dna , nucleoside , irradiation , chemistry , biophysics , combinatorial chemistry , microbiology and biotechnology , biology , biochemistry , physics , nuclear physics
We describe a novel reversible DNA interstrand photocrosslinking reaction via the artificial nucleoside (X). Oligodeoxynucleotide (ODN) containing X can be photocrosslinked by irradiation at 366 nm for 1 s, and the photocrosslinked ODN can be split by irradiation at 312 nm for 60 s.
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