Design of an ultimate quencher free molecular beacon containing pyrrolocytidine-guanine base pair | Zendy

Yoshio Saitō | Zendy; Yoshiaki Shinohara | Zendy; S. S. Bag | Zendy; Yoshiki Takeuchi | Zendy; Katsuhiko Matsumoto | Zendy; Isao Saito | Zendy

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Design of an ultimate quencher free molecular beacon containing pyrrolocytidine-guanine base pair

Author(s) -

Yoshio Saitō,

Yoshiaki Shinohara,

S. S. Bag,

Yoshiki Takeuchi,

Katsuhiko Matsumoto,

Isao Saito

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn182

Subject(s) - molecular beacon , guanine , fluorophore , dna , chemistry , base pair , base (topology) , terminal (telecommunication) , biophysics , fluorescence , biochemistry , nucleotide , biology , physics , computer science , optics , oligonucleotide , telecommunications , gene , mathematical analysis , mathematics

A novel quencher free molecular beacon was designed in which fluorophore-labelled pyrrolocytidine was placed away from the stem terminal. This new type of MB was used for the detection of a target DNA with an excellent efficiency.

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