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Significant alterations in thermal stability of parallel DNA triplexes and antiparallel duplexes were observed upon changing the attachment of ethynylpyrenes from para to ortho in the structure of phenylmethylglycerol inserted as a bulge into DNA (TINA). Insertions of two ortho-TINAs as a pseudo-pair in the middle of the duplex resulted in formation of an excimer band at 505 nm for both 1- and 4-ethynylpyrene analogues.

nucleic acids symposium series

DNA Glue: 1-, 2- and 4-Ethynylpyrenes in the Structure of Twisted Intercalating Nucleic Acids (TINAs), DNA Duplexes/Triplexes and Interstrand Excimer Formation