Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications | Zendy

Diana C. Knapp | Zendy; Angelika Keller | Zendy; Jennifer D’Onofrio | Zendy; Arvydas Lubys | Zendy; Saulius Serva | Zendy; Ants Kurg | Zendy; Maido Remm | Zendy; Marek Kwiatkowski | Zendy; J. W. Engels | Zendy

Open Access

Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications

Author(s) -

Diana C. Knapp,

Angelika Keller,

Jennifer D’Onofrio,

Arvydas Lubys,

Saulius Serva,

Ants Kurg,

Maido Remm,

Marek Kwiatkowski,

J. W. Engels

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn174

Subject(s) - linker , nucleotide , fluoride , chemistry , cleavage (geology) , blocking (statistics) , reagent , fluorescence , fluorescein , combinatorial chemistry , biochemistry , microbiology and biotechnology , biology , gene , organic chemistry , computer science , inorganic chemistry , paleontology , computer network , physics , quantum mechanics , fracture (geology) , operating system

Reversible terminators having a fluoride cleavable 3'-O-blocking group are presented. Each nucleotide triphosphate is labelled by a fluorescent dye cleavable by the same reagent. We present here their synthesis, cleavage experiments and polymerase incorporation tests for a possible use in a process of Sequencing-by-Synthesis.

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