Open AccessOptimized Fluoride Ions Treatment for Release of Base-Sensitive 2'-O-Modified Oligoribonucleotides from Various Solid Supports
Author(s) -
Thomas Lavergne,
Françoise Debart,
JeanJacques Vasseur
Publication year - 2008
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrn162
Subject(s) - fluoride , base (topology) , combinatorial chemistry , chemistry , ion , materials science , inorganic chemistry , organic chemistry , mathematics , mathematical analysis
To synthesize oligoribonucleotides containing base-sensitive 2'-O-modifications, we used the Q-linker or a silyl linker anchored to CPG or polystyrene resin. A fluoride ions treatment was optimized to release oligoribonucleotides from the solid-support and to remove TBDMS groups without affecting 2'-O-acyloxymethyl groups.
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