Development of a novel selective detection method for 5-formyl-2'-deoxyuridine by fluorescent derivatization | Zendy

Wataru Hirose | Zendy; Kousuke Sato | Zendy; Akira Matsuda | Zendy

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Development of a novel selective detection method for 5-formyl-2'-deoxyuridine by fluorescent derivatization

Author(s) -

Wataru Hirose,

Kousuke Sato,

Akira Matsuda

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn141

Subject(s) - derivatization , fluorescence , reagent , deoxyuridine , chemistry , absorbance , combinatorial chemistry , thymidine , chromatography , dna , biochemistry , high performance liquid chromatography , organic chemistry , physics , quantum mechanics

5-Formyl-2'-deoxyuridine (fdUrd) is derived from the oxidation of thymidine and is known to induce mutation (A:T to G:C) in DNA. We planned to develop a novel selective detection method for fdUrd in oligodeoxynucleotide (ODN) based on specific fluorescent derivatization of fdUrd using 2-aminothiophenol derivatives (ATs) as fluorogenic reagents. To achieve this goal, we first synthesized 5-(benzothiazol-2-yl)-2'-deoxyuridine derivatives (btdUrds) via the reaction between fdUrd and 5-substituted ATs. We report herein the synthesis and UV absorbance and fluorescence properties of btdUrds in different pH conditions.

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