Open AccessSensitive DNA probe for photochemical ligation prepared in click chemistry
Author(s) -
Takehiro Ami,
Hiroshi Matsumura,
Kenzo Fujimoto
Publication year - 2008
Publication title -
nucleic acids symposium series
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrn125
Subject(s) - cycloaddition , click chemistry , triazole , chemistry , photochemistry , combinatorial chemistry , dna , 1,3 dipolar cycloaddition , catalysis , organic chemistry , biochemistry
We report a new photosensitive probe, that takes advantage of the electronic structural changes associated with the triazole ring formed in the Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition. We succeeded in performing photoligation on the second time scale. Huisgen cycloaddition are employed to various labeling. As an example, we report a procedure for preparing the DNA probe for quick SNP typing.
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