Stability and Mismatch Discrimination of DNA duplexes containing 2,6-Diaminopurine and 2-Thiothymidine Locked Nucleic Acid bases | Zendy

Curtis Hughesman | Zendy; Robin F. B. Turner | Zendy; Charles A. Haynes | Zendy

Open Access

Stability and Mismatch Discrimination of DNA duplexes containing 2,6-Diaminopurine and 2-Thiothymidine Locked Nucleic Acid bases

Author(s) -

Curtis Hughesman,

Robin F. B. Turner,

Charles A. Haynes

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn124

Subject(s) - oligonucleotide , nucleic acid , differential scanning calorimetry , dna , base pair , chemistry , analytical chemistry (journal) , biochemistry , chromatography , thermodynamics , physics

Hybridization thermodynamics measured by differential scanning calorimetry (DSC) and UV spectroscopy (UVM) are reported for 8- and 14-mer oligonucleotides containing two new LNA bases: 2,6 diaminopurine (D) and 2-thiothymidine (2sT). Oligonucleotides containing D or 2sT bases are shown to have enhanced stability and improved discrimination for several of the possible mismatched base pairs.

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