Open AccessSynthesis of a perylene diimide derivative having two ferrocene moieties as an electrochemical indicator for human telomeric DNA tetraplex
Author(s) -
Ryou Fujikawa,
S. Watanabe,
Keiichi Ohtsuka,
Shinobu Sato,
Shigeori Takenaka
Publication year - 2008
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrn122
Subject(s) - diimide , perylene , ferrocene , electrochemistry , derivative (finance) , electrolyte , dna , chemistry , horizontal scan rate , electrode , polymer chemistry , photochemistry , nuclear chemistry , cyclic voltammetry , organic chemistry , molecule , biochemistry , economics , financial economics
A water-soluble perylene diimide derivative carrying two ferrocene moieties, FPD, was synthesized in two steps as an electrochemical indicator for human telomeric DNA tetraplex. Spectrophotometric binding studies of FPD with AG(3)(T(2)AG(3))(3) revealed its specific binding to tetraplex DNA in 10 mM MES and 1 mM EDTA (pH 6.25) containing 0.10 M KCl. Cyclic voltammograms of AAT CCG TCG AGC AGA G(T(2)AG(3))(4) in an electrolyte containing 20 mM FPN showed single oxidation and reduction peaks based on the ferrocene moieties. The peak current for the oxidation process increased linearly with the scan rate, suggesting the effective concentration of FPN on this electrode.
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