Open AccessSubsites for substrate recognition by bacterial ribonuclease P
Author(s) -
Atsuko Fujimoto,
Sayuri Suwa,
Yu Nagai,
Yo Kikuchi,
Takashi Tanaka
Publication year - 2008
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrn105
Subject(s) - ribozyme , ribonuclease , transfer rna , substrate (aquarium) , substrate specificity , chemistry , biochemistry , rna , enzyme , biology , ecology , gene
We have prepared series of shape variant RNAs of a tRNA precursor and analyzed the substrate shape recognition by bacterial ribonuclease P ribozyme and holoenzyme. The results showed the evidence for the presence of subsites for the recognition of the trna shape. We will discuss a new model for substrate recognition and the role of the protein component.
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