Nitroxide Spin labeled RNA for long range distance measurements by EPR-PELDOR | Zendy

Olga Frolow | Zendy; Burkhard Endeward | Zendy; Olav Schiemann | Zendy; Thomas F. Prisner | Zendy; J. W. Engels | Zendy

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Nitroxide Spin labeled RNA for long range distance measurements by EPR-PELDOR

Author(s) -

Olga Frolow,

Burkhard Endeward,

Olav Schiemann,

Thomas F. Prisner,

J. W. Engels

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn078

Subject(s) - electron paramagnetic resonance , nitroxide mediated radical polymerization , spin (aerodynamics) , nuclear magnetic resonance , range (aeronautics) , rna , physics , materials science , chemistry , biochemistry , composite material , polymerization , thermodynamics , gene , polymer , radical polymerization

Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.

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