Tyrosine-modified PEI: A novel and highly efficient vector for siRNA delivery in mammalian cells | Zendy

Gaëlle Creusat | Zendy; Guy Zuber | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Tyrosine-modified PEI: A novel and highly efficient vector for siRNA delivery in mammalian cells

Author(s) -

Gaëlle Creusat,

Guy Zuber

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn047

Subject(s) - polyethylenimine , small interfering rna , gene delivery , tyrosine , chemistry , transfection , cationic polymerization , rna interference , cell culture , genetic enhancement , cell , rna , vector (molecular biology) , microbiology and biotechnology , biochemistry , biology , gene , recombinant dna , genetics , polymer chemistry

Delivery of synthetic small interfering RNA (siRNA) into cell remains the major obstacle to its biological activity. Cationic polymers, and in particular the "proton sponge" polyethylenimine (PEI), has shown promise for cancer gene therapy but appears inefficient for siRNA delivery. Here we report that modifying branched PEI with amino acids led to efficient siRNA delivery into mammalian cell lines, even in the presence of serum, and at dose as low as 1 nM.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research