Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing | Zendy

M. C. Siomi | Zendy; Haruhiko Siomi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing

Author(s) -

M. C. Siomi,

Haruhiko Siomi

Publication year - 2008

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrn030

Subject(s) - argonaute , microrna , rna , epitope , gene silencing , jurkat cells , small interfering rna , small rna , endogeny , rna silencing , trans acting sirna , biology , computational biology , rna induced silencing complex , microbiology and biotechnology , rna interference , genetics , gene , antibody , t cell , biochemistry , immune system

Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave in respect to small RNA association and target RNA destruction has remained unclear. Recently, we produced monoclonal antibodies for individual hAgos and characterized small RNAs specifically associated with hAgo2 and hAgo3 endogenously expressed in Jurkat cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research