Monitoring of Eco RI-catalyzed cleavage reaction of fluorescent-labeled heterochiral DNA | Zendy

Hidehito Urata | Zendy; Chihiro Tamaki | Zendy; Michiyo Matsuno | Zendy; Shigeo Wada | Zendy; M. Akagi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Monitoring of Eco RI-catalyzed cleavage reaction of fluorescent-labeled heterochiral DNA

Author(s) -

Hidehito Urata,

Chihiro Tamaki,

Michiyo Matsuno,

Shigeo Wada,

M. Akagi

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm227

Subject(s) - molecular beacon , cleavage (geology) , fluorescence , chemistry , catalysis , dna , restriction enzyme , endonuclease , photochemistry , combinatorial chemistry , stereochemistry , biochemistry , oligonucleotide , biology , quantum mechanics , fracture (geology) , paleontology , physics

We have found the unusual reactivity of a heterochiral oligodeoxynucleotide toward restriction endonuclease Eco RI. To conduct the kinetic analysis of the reaction, fluorescent-labeled single-stranded oligodeoxynucleotide molecular beacons were designed and synthesized. The beacons showed a remarkable fluorescence response by addition of Eco RI. The results promise that the beacon could be an effective tool for the kinetic analysis of Eco RI-catalyzed cleavage reaction of the heterochiral oligodeoxynucleotide.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research