Delivery of dsRNA with lactic acid bacteria for RNA interference | Zendy

Akira Kuwahara | Zendy; Machiko Arita | Zendy; Masahiko Sisido | Zendy; Takashi Ohtsuki | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Delivery of dsRNA with lactic acid bacteria for RNA interference

Author(s) -

Akira Kuwahara,

Machiko Arita,

Masahiko Sisido,

Takashi Ohtsuki

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm207

Subject(s) - rna interference , small hairpin rna , rna silencing , lactobacillus casei , rna , biology , microbiology and biotechnology , blot , gene expression , gene , bacteria , biochemistry , genetics

RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research