Non-sequence specific RNA inhibition of HIV-1 replication | Zendy

Kimiko Kato | Zendy; Yoshiki Habu | Zendy; Hiroaki Takaku | Zendy

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Non-sequence specific RNA inhibition of HIV-1 replication

Author(s) -

Kimiko Kato,

Yoshiki Habu,

Hiroaki Takaku

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm206

Subject(s) - rna , dicer , rna silencing , rnase h , microbiology and biotechnology , rna editing , biology , nuclease protection assay , rna dependent rna polymerase , rnase p , subgenomic mrna , non coding rna , rna interference , virology , chemistry , genetics , gene

In this study, we investigated an RNA (R-Psi-sgRNA) that suppresses HIV-1 replication. This RNA is expressed by a plasmid vector (pR-Psi-sgRNA-ter) that was constructed accidentally. To examine if this effect is caused by RNA interference, R-Psi-sgRNA was synthesized in vitro and treated with the Dicer enzyme, an important RNase III enzyme for RNA interference. The RNA was cleaved into fragments of approximately 20 nucleotides. We then performed an HIV-1 p24 assay with the RNA fragments to evaluate their effect on HIV-1 replication. HIV-1 replication was suppressed. We are now analyzing the sequences of the RNA fragments.

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