Selection and characterization of DNA aptamers against VEGF165 with aptamer blotting method and its application | Zendy

Kazunori Ikebukuro | Zendy; Hiroo Hasegawa | Zendy; Koji Sode | Zendy

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Selection and characterization of DNA aptamers against VEGF165 with aptamer blotting method and its application

Author(s) -

Kazunori Ikebukuro,

Hiroo Hasegawa,

Koji Sode

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm200

Subject(s) - aptamer , systematic evolution of ligands by exponential enrichment , blot , dna , selection (genetic algorithm) , selex aptamer technique , microbiology and biotechnology , computational biology , target protein , biology , genetics , computer science , gene , rna , artificial intelligence

We have isolated two DNA aptamers against a tumor marker protein, human vascular endothelial growth factor (VEGF165). To select aptamers that have high specificity for VEGF165, we used another protein as a competitor in the SELEX process. In addition, we evaluated the screened library with aptamer blotting. Isolated aptamers bound to VEGF165 with Kd in a few hundred-nanomolar ranges, and did not bind to a competitor. This selection method allowed us to select the specific aptamers against a target protein efficiently. Furthermore, isolated aptamers did not bind to VEGF121. These specific DNA aptamers against VEGF165 would be a useful sensing element for cancer diagnosis.

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