Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme | Zendy

Tatsuki Tanaka | Zendy; Yo Kikuchi | Zendy

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Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme

Author(s) -

Tatsuki Tanaka,

Yo Kikuchi

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm186

Subject(s) - ribozyme , rnase p , escherichia coli , rnase h , rna , ribonuclease , chemistry , ribonuclease iii , domain (mathematical analysis) , computational biology , biochemistry , biology , microbiology and biotechnology , gene , mathematical analysis , mathematics , rna interference

We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.

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