Structural characterization of supercoiled DNA containing a minisatellite repeat that has polypurine/polypyrimidine stretch | Zendy

Mikio Kato | Zendy; Takeshi Haku | Zendy; Takahiro Yamauchi | Zendy; Shigeru Sugiyama | Zendy; Kuniaki Nagayama | Zendy; Nobutaka Shimizu | Zendy

Open Access

Structural characterization of supercoiled DNA containing a minisatellite repeat that has polypurine/polypyrimidine stretch

Author(s) -

Mikio Kato,

Takeshi Haku,

Takahiro Yamauchi,

Shigeru Sugiyama,

Kuniaki Nagayama,

Nobutaka Shimizu

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm171

Subject(s) - puc19 , dna supercoil , dna , plasmid , tandem repeat , biophysics , sequence (biology) , chemistry , cruciform , molecule , minisatellite , repeated sequence , microbiology and biotechnology , biology , biochemistry , materials science , microsatellite , gene , genome , composite material , dna replication , allele , organic chemistry

The pUC19 derivative pAL79 contains six tandem repeats of a 30-bp unit sequence. Supercoiled pAL79 molecules show aberrant electrophoretic mobility under acidic conditions, and microscopic analysis suggests pAL79 molecules conform to tight plectonemes when deposited on a substrate under acidic conditions. This may mean that a short inserted DNA sequence affects the global topology of plasmid DNA.

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