Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers | Zendy

Naohiko Shimada | Zendy; Kunio Muraki | Zendy; Toyoaki Anai | Zendy; Arihiro Kano | Zendy; Atsushi Maruyama | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers

Author(s) -

Naohiko Shimada,

Kunio Muraki,

Toyoaki Anai,

Arihiro Kano,

Atsushi Maruyama

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm170

Subject(s) - cationic polymerization , dna , nucleotide , microbiology and biotechnology , betaine , copolymer , single nucleotide polymorphism , base pair , single strand , genetics , chemistry , biology , biophysics , biochemistry , polymer chemistry , gene , polymer , genotype , organic chemistry

We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research