Electrochemical RNase detection using ferrocenylnaphthalene diimide

To develop a conventional RNase detecting system, an RNA electrode was constructed by the immobilization of poly(A)+RNA from mouse kidney on the glassy carbon electrode. Electrochemical measurement using this RNA electrode in the electrolyte containing ferrocenylnaphthalene diimide (1) was carried out and showed the electrochemical signal depending on the amount of the immobilized RNA. After this electrode was treated with water, the differential pulse voltammetric (DPV) measurement was subsequently conducted in the electrolyte containing 1. When RNase is contained in the water, the electrochemical signal decreased with an increase of the amount of RNase. This is derived from the decreasing amount of RNA on the electrode by RNase. In DPV measurement, the concentration of RNaseA was linear in the range of 10(-10) - 10(-8) M and the amount of RNase in the tap water could be estimated.