Open AccessPhotochemical properties of novel fluorescent probes for detection of DNA
Author(s) -
Shuji Ikeda,
Akimitsu Okamoto
Publication year - 2007
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrm151
Subject(s) - fluorescence , oligomer , dna , chemistry , photochemistry , orange (colour) , thiazole , biophysics , biochemistry , biology , stereochemistry , organic chemistry , optics , physics , food science
A variety of fluorescence-labeled DNA oligomers have been developed for gene analysis. We have studied on the development of a new functional fluorescent DNA probe for decrease in false positive signals. Thiazole orange derivatives were designed for the fluorescent label of DNA oligomers. The DNA oligomer containing a thiazole orange dye exhibited fluorescence with a higher intensity on hybridization with a complementary RNA, compared with that from the oligomer alone.
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