Detection of one base mismatch using photochemical ligation | Zendy

Masashi Mukae | Zendy; Miyuki Tabara | Zendy; Paola Arslan | Zendy; Toshihiro Ihara | Zendy; Akinori Jyo | Zendy

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Detection of one base mismatch using photochemical ligation

Author(s) -

Masashi Mukae,

Miyuki Tabara,

Paola Arslan,

Toshihiro Ihara,

Akinori Jyo

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm144

Subject(s) - anthracene , conjugate , chemistry , covalent bond , reactivity (psychology) , ligation , photochemistry , yield (engineering) , molecule , selectivity , sequence (biology) , base pair , fluorescence , combinatorial chemistry , dna , organic chemistry , materials science , biochemistry , biology , catalysis , microbiology and biotechnology , medicine , mathematical analysis , alternative medicine , mathematics , physics , pathology , quantum mechanics , metallurgy

We attached anthracene molecules covalently to one end of oligodeoxyribonucleotides to form three pairs of anthracene-ODN conjugates. The sequences of each pair of the conjugates were designed to bind adjacent sequences of the template with the anthracene units directed such that they stacked each other. The conjugates dimerized only in the presence of the template by light irradiation. We found that the ligation yields largely depended on the substituted position of anthracene groups. Sequence selectivity in the dimerization yield was preserved even in the system with highest reactivity. SNP bases could be discriminated rapidly by combining the techniques of MS spectroscopy.

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