Recognition of target chain-length by use of oligonucleotides having bulky substituents at the terminal bases | Zendy

Yotaro Takaku | Zendy; Masahiro Mizuta | Zendy; A. Ohkubo | Zendy; M. SEKINE | Zendy; Kohji Seio | Zendy

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Recognition of target chain-length by use of oligonucleotides having bulky substituents at the terminal bases

Author(s) -

Yotaro Takaku,

Masahiro Mizuta,

A. Ohkubo,

M. SEKINE,

Kohji Seio

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm142

Subject(s) - oligonucleotide , rna , microrna , duplex (building) , terminal (telecommunication) , chemistry , long non coding rna , chain (unit) , combinatorial chemistry , computational biology , microbiology and biotechnology , stereochemistry , biology , dna , biochemistry , gene , computer science , physics , telecommunications , astronomy

We synthesized 2'-O-methyl RNAs having bulky substituents at the 5' and 3'-terminal bases and examined their hybridization properties. The hybridization of the modified RNAs to the targets RNA was studied varying the chain length of the targets. As the result, it was proved that the duplex of the modified 2'-O-methyl RNAs and the long targets were destabilized in comparison with that of the modified 2'-O-methyl RNAs and the short targets. We hope that these oligonucleotides which could recognize the target chain-length would be the simple and convenient tool for the detection of small RNAs such as the microRNAs.

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