Polymerisation of a DNA strand using oligo-DNA template with modified bases, sugars and phosphates | Zendy

Jun-ichi Nagashima | Zendy; Satoshi Minezaki | Zendy; Satoshi Obika | Zendy; Tadashi Imanishi | Zendy; Masayasu Kuwahara | Zendy; H. Sawai | Zendy

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Polymerisation of a DNA strand using oligo-DNA template with modified bases, sugars and phosphates

Author(s) -

Jun-ichi Nagashima,

Satoshi Minezaki,

Satoshi Obika,

Tadashi Imanishi,

Masayasu Kuwahara,

H. Sawai

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm028

Subject(s) - dna , chemistry , primer (cosmetics) , template , uracil , dna polymerase , dna clamp , coding strand , polymerase , biochemistry , combinatorial chemistry , primer extension , stereochemistry , polymerase chain reaction , organic chemistry , gene , materials science , base sequence , nanotechnology , reverse transcriptase

We have attempted to synthesise a complimentary strand using oligo-DNA with modified bases, sugars and phosphates as a template strand by polymerase reaction. Analogues bearing C5-substituted uracil, those with amide linkage [-CH2C=ONH-] in place of phospho-diester linkage and those bearing 2'-O, 4'-C-bridged sugar were used. Primer extension reactions were carried out to synthesise complimentary DNA strands. The reactions depended on the thermostable DNA polymerase used, the type of modification or the number of the modified position on the template strand.

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