Nucleotide incorporation against 7,8-dihydro-8-oxoguanine is influenced by neighboring base sequences in TLS DNA polymerase reaction | Zendy

Cathleen M. Yung | Zendy; Tetsuya Suzuki | Zendy; Yoshinaga Okugawa | Zendy; Atsushi Kawakami | Zendy; David Loakes | Zendy; Kazuo Negishi | Zendy; Tomoe Negishi | Zendy

Open Access

Nucleotide incorporation against 7,8-dihydro-8-oxoguanine is influenced by neighboring base sequences in TLS DNA polymerase reaction

Author(s) -

Cathleen M. Yung,

Tetsuya Suzuki,

Yoshinaga Okugawa,

Atsushi Kawakami,

David Loakes,

Kazuo Negishi,

Tomoe Negishi

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm025

Subject(s) - transversion , oligonucleotide , dna polymerase , microbiology and biotechnology , polymerase , biology , base pair , sequence (biology) , genetics , dna , dna damage , mutation , chemistry , gene

7,8-Dihydro-8-oxoguanine (8-oxoG) is a well-known oxidative lesion in DNA and is related to carcinogenesis and ageing processes. Misincorporation of dATP opposite to 8-oxoG leads to G --> T transversion mutations. DNA sequence has been proved as an important factor influencing the replication and enzymatic repair of various types of damages. To explore the influence of sequence effect on the properties of translesion synthesis (TLS) polymerase bypass of 8-oxoG, oligonucleotides with an 8-oxoG in different sequence contexts were used. We conclude that the 5'-nearest base next to 8-oxoG has significant effects in the G --> T mutation by hpoleta.

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