Kinetic analysis of the effects of translation enhancers in translation initiation | Zendy

Shuntaro Takahashi | Zendy; Hiroyuki Furusawa | Zendy; Yoshihiro Shimizu | Zendy; Takuya Ueda | Zendy; Yoshio Okahata | Zendy

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Kinetic analysis of the effects of translation enhancers in translation initiation

Author(s) -

Shuntaro Takahashi,

Hiroyuki Furusawa,

Yoshihiro Shimizu,

Takuya Ueda,

Yoshio Okahata

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm023

Subject(s) - translation (biology) , enhancer , ribosome , messenger rna , protein biosynthesis , eukaryotic translation , initiation factor , biology , chemistry , microbiology and biotechnology , biochemistry , rna , gene , gene expression

Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.

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