Molecular mechanism of trans-translation | Zendy

Daisuke Kurita | Zendy; Takayuki Konno | Zendy; Kazuma Takada | Zendy; Akira Muto | Zendy; Hyouta Himeno | Zendy

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Molecular mechanism of trans-translation

Author(s) -

Daisuke Kurita,

Takayuki Konno,

Kazuma Takada,

Akira Muto,

Hyouta Himeno

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm022

Subject(s) - ribosome , transfer rna , translation (biology) , protein biosynthesis , ef tu , stop codon , p site , translation system , chemistry , messenger rna , biology , biochemistry , microbiology and biotechnology , amino acid , genetics , rna , in vitro , gene

mRNA has a dual function as a tRNA and an mRNA to relieve the stalled ribosome. During this process, tmRNA enters the ribosomal A-site without a codonanticodon interaction, but with a protein factor SmpB. Here, we established in vitro trans-translation system, which is able to evaluate peptidyl transfer to alanyltmRNA and translation of the resume codon on tmRNA. Using this system, the effects of mutations on tmRNA or SmpB are assessed by measuring the incorporations of labeled amino acid into polypeptide. Interaction of SmpB with ribosome and tmRNA was studied by directed hydroxyl radical probing. Our results revealed that SmpB constitutes an important element during trans-translation. We propose a new model of transtranslation.

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