Design of dual-labeled oligonucleotide probes for SNPs genotyping | Zendy

Yoshiro Saito | Zendy; S. S. Bag | Zendy; S. Kodate | Zendy; Iwao Suzuka | Zendy

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Design of dual-labeled oligonucleotide probes for SNPs genotyping

Author(s) -

Yoshiro Saito,

S. S. Bag,

S. Kodate,

Iwao Suzuka

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm012

Subject(s) - oligonucleotide , genotyping , typing , fluorescence , snp genotyping , nucleotide , fluorescein , acceptor , base pair , snp , single nucleotide polymorphism , combinatorial chemistry , chemistry , computational biology , microbiology and biotechnology , biology , genetics , dna , genotype , physics , gene , optics , condensed matter physics

Our effort in designing base-discriminating fluorescence nucleosides (BDF), leads us to develop dual-labeled oligonucleotide probe in which the BDF nucleoside, (Py)U/(2-Ant)U act as the donor separated by a defined base pair distance from the acceptor, fluorescein, attached to 5'-end of the probe. Thus, a longer wavelength emission from acceptor might allow the probe to be used for SNP typing in chip based detection technology or in cell.

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