Development of an unnatural base pair for efficient PCR amplification | Zendy

Ichiro Hirao | Zendy; Takeki Mitsui | Zendy; Michiko Kimoto | Zendy; Shigeyuki Yokoyama | Zendy

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Development of an unnatural base pair for efficient PCR amplification

Author(s) -

Ichiro Hirao,

Takeki Mitsui,

Michiko Kimoto,

Shigeyuki Yokoyama

Publication year - 2007

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrm005

Subject(s) - base pair , nucleic acid , dna , genetics , alphabet , biology , base (topology) , microbiology and biotechnology , computational biology , chemistry , mathematics , mathematical analysis , linguistics , philosophy

An unnatural base pair system could expand the genetic alphabet, enabling the site-specific incorporation of extra, functional components into nucleic acids and proteins. We developed an unnatural base pair between 7-(2-thienyl)imidazo[4,5-b]pyridine (denoted by Ds) and 2-nitropyrrole (denoted by Pn), which specifically and efficiently functions in DNA amplification by PCR. After 20 cycles of PCR, the mutation rate of the Ds-Pn pair in an amplified DNA fragment was approximately 1%. The Ds-Pn pair system in combination with other unnatural base pairs could be useful for DNA/RNA-based biotechnology.

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