Open AccessEstimation of DNA polymerase for improvement of rolling circle amplification
Author(s) -
Takeo Yoshimura,
Saori Arikado,
Shokichi Ohuchi
Publication year - 2006
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrl151
Subject(s) - rolling circle replication , polymerase , dna polymerase , primer (cosmetics) , biology , polymerase chain reaction , genetics , applications of pcr , microbiology and biotechnology , gene , dna , computational biology , chemistry , multiplex polymerase chain reaction , organic chemistry
Rolling Circle Amplification (RCA) is a technique to amplify the oligoncleotides containing tandemly repetitive sequence motif. Various repetitive sequences of which the motif unit is the circular template can be synthesized by extending the primer. Additionally, RCA need not change the temperature regulatory processes like PCR, but RCA has the gene amplification efficiency similar to PCR. Although, it contains the problem of the sequence confirmation and the activity of polymerase as the DNA polymerase I. We comparatively estimated of the various polymerases for the improvement of the RCA. Five DNA polymerases were estimated for RCA.
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