Nucleotide insertion opposite a cyclobutane pyrimidine dimer analogue caused from photoligation by a replicative DNA polymerase | Zendy

Masayuki Ogino | Zendy; Daisuke Okamura | Zendy; Yoshinaga Yoshimura | Zendy; Kenzo Fujimoto | Zendy

Open Access

Nucleotide insertion opposite a cyclobutane pyrimidine dimer analogue caused from photoligation by a replicative DNA polymerase

Author(s) -

Masayuki Ogino,

Daisuke Okamura,

Yoshinaga Yoshimura,

Kenzo Fujimoto

Publication year - 2006

Publication title -

nucleic acids symposium series

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/nrl062

Subject(s) - pyrimidine dimer , cyclobutane , nucleotide , dimer , dna polymerase , dna , polymerase , chemistry , pyrimidine , microbiology and biotechnology , stereochemistry , genetics , biology , dna repair , gene , ring (chemistry) , organic chemistry

We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine ((CV)U)-containing ODN at the 5'-terminal. This method forms d(T-(CV)U) as a cyclobutane pyrimidine dimer (CPD) analogue between 3'-terminal thymidine and 5'-terminal (CV)U of two oligodeoxynucleotides (ODNs). In this study, we performed PCR using a DNA template containing d(T-(CV)U). And then, we disclosed that two adenines were incorporated opposite the d(T-(CV)U).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research