Exploration of human miRNA target genes in neuronal differentiation | Zendy

Y. Fukuda | Zendy; Hiroaki Kawasaki | Zendy; Kazunari Taira | Zendy

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Exploration of human miRNA target genes in neuronal differentiation

Author(s) -

Y. Fukuda,

Hiroaki Kawasaki,

Kazunari Taira

Publication year - 2005

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/49.1.341

Subject(s) - microrna , biology , gene , luciferase , untranslated region , translation (biology) , gene expression , three prime untranslated region , regulation of gene expression , microbiology and biotechnology , rna , messenger rna , genetics , transfection

MicroRNAs (miRNAs) are endogenous non-coding RNA molecules that inhibit protein translation in a sequence specific manner. We carried out microarray analyses of 180 human pre-miRNAs in neuroblastoma cell, SH-SY5Y, following stimulation by TPA. Twelve of the pre-miRNAs were up-regulated by the TPA stimulation. We also explored miRNA target genes associated with neuronal differentiation. Some miRNAs have complementarity with 3'UTR of the Notch1 gene, a regulator of neuronal differentiation, and luciferase assay showed that overexpression of these miRNAs reduced the luciferase activity of reporter genes containing the Notch1-3'UTR sequence. Our results suggest that miRNAs can be associated with TPA induced signalling pathways and expression of Notch1 gene.

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