Open AccessDesign of light-switchable phage promoter for efficient photo-regulation of gene-expression
Author(s) -
Mingzhe Liu,
Makoto Komiyama,
Hiroyuki Asanuma
Publication year - 2005
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/49.1.283
Subject(s) - transcription (linguistics) , azobenzene , t7 rna polymerase , promoter , chemistry , rna polymerase ii , rna polymerase , gene , microbiology and biotechnology , gene expression , biophysics , biology , rna , bacteriophage , biochemistry , molecule , linguistics , philosophy , escherichia coli , organic chemistry
Efficient photoregulation of transcription by T7 RNA polymerase (RNAP) has been achieved with modified T7-promoter involving two azobenzene moieties at RNAP recognition region and TATA region simultaneously. Transcription proceeded 7.5-fold faster after UV irradiation than that under dark condition. Kinetic parameters (kcat, Km) for the transcription revealed that an azobenzene at each position played a different role and thus synergistic effect of photo-regulation was achieved with the promoter involving two azobenzenes.
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