Open AccessCoformational Switch of Oligonucleotide Induced by Spermine
Author(s) -
Toshimasa Kirihata,
Shuichi Nakano,
Satoshi Fujii,
Manabu Matsumoto,
Masayasu Kuwahara,
Hiroaki Sawai,
Naoki Sugimoto
Publication year - 2005
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/49.1.241
Subject(s) - spermine , nucleic acid , oligonucleotide , chemistry , dna , duplex (building) , dissociation (chemistry) , melting temperature , conformational change , nucleic acid structure , biochemistry , combinatorial chemistry , stereochemistry , biophysics , rna , biology , organic chemistry , materials science , enzyme , composite material , gene
Conformational change of nucleic acids induced by a small molecule is essential for many biological reactions and useful for biotechnology. To construct a spermine-responsive nucleic acid, we designed DNA sequences which can potentially form a unimolecular hairpin loop structure and a bimolecular duplex by itself. On the basis of UV melting and native PAGE experiments, we found four DNA oligomers of d16cg, d14cg, d12cg, and d14ta adopting different secondary structures depending on the spermine concentration. Their dissociation constants (Kd) with spermine were estimated in the order of 10(-6).
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