Open AccessSpectroscopic analyses of DNA duplexes in the presence of mercury ions
Author(s) -
Hiroshi Yamaguchi,
Shuji Oda,
Chojiro Kojima,
Akira Ono,
Yoshinori Kondo,
Yoshiyuki Tanaka
Publication year - 2005
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/49.1.199
Subject(s) - two dimensional nuclear magnetic resonance spectroscopy , chemistry , duplex (building) , ion , mercury (programming language) , dna , nuclear magnetic resonance spectroscopy , spectroscopy , crystallography , stereochemistry , base pair , proton nmr , spectral line , physics , biochemistry , quantum mechanics , astronomy , organic chemistry , computer science , programming language
The DNA duplex with tandem T x T mismatches in the presence of Hg(II) has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches (TT10). From two-dimensional (2D) 1H-1H NOESY spectrum of TT10 in complex with Hg(II), we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all of them. Based on these assignments and NOESY spectra, we could assigned all the non-exchangeable protons of TT10 in the presence of Hg(II).
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