Open AccessNile Red nucleoside : Novel nucleoside analog with a fluorophore replacing the DNA base
Author(s) -
Kazuki Tainaka,
Yoshimasa Fujiwara,
Akimitsu Okamoto
Publication year - 2005
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/49.1.155
Subject(s) - solvatochromism , nile red , fluorophore , nucleoside , chromophore , fluorescence , chemistry , polarity (international relations) , photochemistry , dna , solvent , stereochemistry , biochemistry , optics , physics , cell
A solvatochromic dye is very useful in studies of local polarity and dynamics in biological systems. A novel solvatochromic chromophore, Nile Red beta-C-2'-deoxyriboside, has been synthesized to act as a photophysical probe, and incorporated site-selectively into an oligodeoxynucleotide. The absorption and fluorescence spectra of Nile Red nucleoside showed a remarkable sensitivity to the solvent polarity.
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