Discrimination of the length of two remote binding sites by the spacer-linked DNA minor groove binders | Zendy

Mikimasa Tanada | Zendy; Shigeki Sasaki | Zendy

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Discrimination of the length of two remote binding sites by the spacer-linked DNA minor groove binders

Author(s) -

Mikimasa Tanada,

Shigeki Sasaki

Publication year - 2005

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/49.1.1

Subject(s) - dna , groove (engineering) , surface plasmon resonance , minor groove , duplex (building) , base pair , molecule , binding site , chemistry , crystallography , biophysics , stereochemistry , nanotechnology , biology , materials science , biochemistry , organic chemistry , nanoparticle , metallurgy

The aim of this study is to develop new recognition molecules for the DNA higher-ordered structure on the basis of new concept of DNA recognition mode. In order to bind two remote binding sites of DNA at the same time, we designed the new bidentate ligands that have two Hoechst 33258 units connected by a spacer. DNA binding affinity of the ligands was evaluated by measuring SPR (surface plasmon resonance). As a result, we have achieved discrimination of the length of two remote binding region of a DNA duplex.

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