Open AccessScreening of modified DNA aptamers that recognize DNA secondary structure
Author(s) -
Masayasu Kuwahara
Publication year - 2004
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/48.1.265
Subject(s) - dna , aptamer , base pair , dna polymerase , combinatorial chemistry , computational biology , dna clamp , chemistry , polymerase , microbiology and biotechnology , biology , polymerase chain reaction , biochemistry , gene , reverse transcriptase
We attempted to utilize in vitro selection techniques to develop modified DNA aptamers that are specific for DNA secondary structure including a single-mismatch base pair. For the selection, two kinds of random modified DNA library were prepared by an enzymatic method using modified 2'-deoxynucleoside 5'-triphosphates and KOD Dash DNA polymerase: one library consists of modified DNA including amino functionality and another consists of modified DNA including both hydroxypropynyl and guanidium functionalities.
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