Open AccessDevelopment of a novel DNA sensing system using DNA aptamer inhibited enzymatic activity 1
Author(s) -
Kazunori Ikebukuro
Publication year - 2004
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/48.1.231
Subject(s) - aptamer , adapter (computing) , dna , microbiology and biotechnology , chemistry , a dna , hybridization probe , thrombin , biochemistry , biology , computer science , platelet , immunology , operating system
A novel DNA detection system has been developed using the DNA aptamer inhibiting thrombin activity. The complementary strand to the target DNA was inserted into the loop region of the G-quartet structure of this adapter aiming at the disruption of it by the hybridization of the target DNA with its complementary strand in the aptamer. Using this modified aptamer, we were able to detect the target DNA by measuring thrombin activity.
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